storm imaging human dermal fibroblasts hdfs  (ATCC)

Journal: Plants

Article Title: Ginsenoside-Enriched Panax ginseng Sprouts Cultivated from Aquaponic System with a Novel Nutrient Solution Regulate LPS-Induced Inflammatory Cytokines and UVB-Induced Photoaging Responses via MAPK/AP-1 Signaling Pathways

doi: 10.3390/plants14111712

Figure Lengend Snippet: Effects of ginseng extracts (GE), the extract of kelp fermentates−treated ginseng (FGE), the water fraction of crude saponin extract from FGE (WFGE) and the 70% ethanol fraction of crude saponin extract from FGE (EFGE) on cell viability ( A , B ), procollagen ( C ) and MMP−1 ( D ), and the expressions of MMP−1 and MMP−3 ( E ) in UVB−irradiated Hs68 cells. Data values are expressed as mean as S.D. of triplicate determinations. Significant differences were compared with control at * p < 0.05, ** p < 0.01, and *** p < 0.001, and with the UVB group at ## p < 0.01 and ### p < 0.001 by one−way analysis of variance and Tukey’s multiple comparison.

Article Snippet: Mouse macrophage RAW264.7 cells and human dermal fibroblast Hs68 cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Irradiation, Control, Comparison

Journal: Plants

Article Title: Ginsenoside-Enriched Panax ginseng Sprouts Cultivated from Aquaponic System with a Novel Nutrient Solution Regulate LPS-Induced Inflammatory Cytokines and UVB-Induced Photoaging Responses via MAPK/AP-1 Signaling Pathways

doi: 10.3390/plants14111712

Figure Lengend Snippet: Effects of ginseng extracts (GE), the extract of kelp fermentates−treated ginseng (FGE), the water fraction of crude saponin extract from FGE (WFGE) and the 70% ethanol fraction of crude saponin extract from FGE (EFGE) on expressions of mitogen−activated protein kinases ( A ) and activator protein−1 ( B ) signaling pathways in UVB−irradiated Hs68 cells. Data values are expressed as mean as S.D. of triplicate determinations. Significant differences were compared with control at * p < 0.05, ** p < 0.01, and *** p < 0.001 and with UVB group at ### p < 0.001 by one−way analysis of variance and Tukey’s multiple comparison.

Article Snippet: Mouse macrophage RAW264.7 cells and human dermal fibroblast Hs68 cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Protein-Protein interactions, Irradiation, Control, Comparison

Journal: Antioxidants

Article Title: 10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

doi: 10.3390/antiox13121547

Figure Lengend Snippet: Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

Article Snippet: The B16F10 murine melanoma cell line and Hs68 human dermal fibroblast cell line were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay, Control, Positive Control

Journal: Microorganisms

Article Title: Targeting Inflammation and Skin Aging via the Gut–Skin Axis: The Role of Lactiplantibacillus plantarum HY7714-Derived Extracellular Vesicles

doi: 10.3390/microorganisms12122466

Figure Lengend Snippet: Protective effect of Lpb. plantarum HY7714 EVs on HS68 cells. ( A ) Effect of different concentrations (25, 50, and 100 μg/mL) of HY7714 EVs on viability. ( B – D ) Effect of HY7714 EVs on ECM-related gene expressions. Data are presented as the mean ± SE. Significant differences are indicated by * p < 0.05, ** p < 0.01, and *** p < 0.001 relative to the control group. Significant differences are indicated by # p < 0.05 and ### p < 0.001 relative to the TS group. SE, standard error; CONT, control; EVs, extracellular vesicles; TS EVs, Lpb. plantarum type strain KCTC3180 EVs; HY7714 EVs, Lpb. plantarum HY7714 EVs.

Article Snippet: Human HT-29 and HS68 (CRL-1635) human dermal fibroblasts were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control