Cell Culture:Article Title: The Potential Anti-Photoaging Effect of Photodynamic Therapy Using Chlorin e6-Curcumin Conjugate in UVB-Irradiated Fibroblasts and Hairless Mice
Article Snippet: .. Hs68 human dermal fibroblasts were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in monolayers at 37 °C in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum. .. Hs68 cells (2 × 10 5 cells/well) were seeded in a 6-well plate (Falcon, Corning, NY, USA) for 24 h, and then treated with 7 and 10 nM Ce6-PEG-Cur for 30 min at 37 °C in an atmosphere of 5% CO 2 in the dark after UVB (200 mJ/cm 2 ) irradiation.
Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1
Article Snippet: .. HaCaT keratinocytes (AddexBio, San Diego, CA, USA; T0020001), HS68 human dermal fibroblasts (ATCC, Manassas, VA, USA; CRL-1635), and Raw264.7 murine macrophages (KCLB, Seoul, Korea; 40071) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS; Gibco) and 1 % penicillin-streptomycin (Gibco) at 37 °C in a humidified 5 % CO 2 incubator. .. Human umbilical vein endothelial cells (HUVECs; Lonza, Walkersville, MD, USA) were maintained in Endothelial Cell Growth Medium (EGM; Lonza) under identical conditions.
Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1
Article Snippet: .. HaCaT keratinocytes (AddexBio, San Diego, CA, USA; T0020001), HS68 human dermal fibroblasts (ATCC, Manassas, VA, USA; CRL-1635), and Raw264.7 murine macrophages (KCLB, Seoul, Korea; 40071) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS; Gibco) and 1 % penicillin-streptomycin (Gibco) at 37 ◦C in a humidified 5 % CO2 incubator. .. Human umbilical vein endothelial cells (HUVECs; Lonza, Walkersville, MD, USA) were maintained in Endothelial Cell Growth Medium (EGM; Lonza) under identical conditions.
Article Title: Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells
Article Snippet: .. Hs68 human dermal fibroblasts (American Type Culture Collection (ATCC), Manassas, VA, USA) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (Hyclone, Logan, UT, USA) supplemented with 10% of fetal bovine serum (Hyclone) and 1% penicillin-streptomycin. ..
Modification:Article Title: The Potential Anti-Photoaging Effect of Photodynamic Therapy Using Chlorin e6-Curcumin Conjugate in UVB-Irradiated Fibroblasts and Hairless Mice
Article Snippet: .. Hs68 human dermal fibroblasts were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in monolayers at 37 °C in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum. .. Hs68 cells (2 × 10 5 cells/well) were seeded in a 6-well plate (Falcon, Corning, NY, USA) for 24 h, and then treated with 7 and 10 nM Ce6-PEG-Cur for 30 min at 37 °C in an atmosphere of 5% CO 2 in the dark after UVB (200 mJ/cm 2 ) irradiation.
Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1
Article Snippet: .. HaCaT keratinocytes (AddexBio, San Diego, CA, USA; T0020001), HS68 human dermal fibroblasts (ATCC, Manassas, VA, USA; CRL-1635), and Raw264.7 murine macrophages (KCLB, Seoul, Korea; 40071) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS; Gibco) and 1 % penicillin-streptomycin (Gibco) at 37 °C in a humidified 5 % CO 2 incubator. .. Human umbilical vein endothelial cells (HUVECs; Lonza, Walkersville, MD, USA) were maintained in Endothelial Cell Growth Medium (EGM; Lonza) under identical conditions.
Article Title: Wharton's jelly mesenchymal stem cell-secretome enhances skin rejuvenation via ApoA4 and SERPINH1
Article Snippet: .. HaCaT keratinocytes (AddexBio, San Diego, CA, USA; T0020001), HS68 human dermal fibroblasts (ATCC, Manassas, VA, USA; CRL-1635), and Raw264.7 murine macrophages (KCLB, Seoul, Korea; 40071) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS; Gibco) and 1 % penicillin-streptomycin (Gibco) at 37 ◦C in a humidified 5 % CO2 incubator. .. Human umbilical vein endothelial cells (HUVECs; Lonza, Walkersville, MD, USA) were maintained in Endothelial Cell Growth Medium (EGM; Lonza) under identical conditions.
Article Title: Nonanal Stimulates Growth Factors via Cyclic Adenosine Monophosphate (cAMP) Signaling in Human Hair Follicle Dermal Papilla Cells
Article Snippet: .. Hs68 human dermal fibroblasts (American Type Culture Collection (ATCC), Manassas, VA, USA) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (Hyclone, Logan, UT, USA) supplemented with 10% of fetal bovine serum (Hyclone) and 1% penicillin-streptomycin. ..
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